Probiotic Studies in Neonatal Mice Using Gavage

Freddy Francis; Natallia Varankovich; Byron Brook; Nelly Amenyogbe; Rym Ben-Othman; Bing Cai; Danny Harbeson; Aaron  C. Liu; Ben Dai; Shelly McErlane; Kris Andrews; Tobias  R. Kollmann; Pinaki Panigrahi

doi:10.3791/59074

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免疫与感染

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Probiotic Studies in Neonatal Mice Using Gavage

JoVE 杂志
免疫与感染
Author Produced

This content is Free Access.

JoVE 杂志免疫与感染

Probiotic Studies in Neonatal Mice Using Gavage

DOI:

10.3791/59074-v

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10:36 min

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January 27, 2019

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Freddy Francis, Natallia Varankovich, Byron Brook, Nelly Amenyogbe, Rym Ben-Othman, Bing Cai, Danny Harbeson, Aaron C. Liu, Ben Dai, Shelly McErlane, Kris Andrews, Tobias R. Kollmann*^1,2, Pinaki Panigrahi*³

¹Department of Experimental Medicine,University of British Columbia, ²Department of Pediatrics,University of British Columbia, ³Department of Epidemiology and Pediatrics,University of Nebraska Medical Centre, ⁴Animal Care Services,University of British Columbia

Chapters

00:00Title
00:04Intra-esophageal Gavage of Neonatal Mouse – Day of Life 2
05:19Collection of Intestinal Samples for Colonization Analysis
08:38Lactobacillus plantarum Colonization with Gavage Everyday or Every Other Day
09:03Lactobacillus plantarum Gavaged Mice Co-housed with Untreated Mice
09:28Conclusion

Summary

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This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include but is not limited to probiotic dosing, methods of administration, and quantification of bacteria in the intestines.

Probiotic Studies in Neonatal Mice Using Gavage

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