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Immunologie et infection
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In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
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Immunologie et infection
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Journal JoVE Immunologie et infection
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

DOI:
10.3791/58929-v

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10:26 min

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January 20, 2019

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, , , ,
1IGH, CNRS,Univ Montpellier, 2Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology,CHU Montpellier, 3UFR de Médecine,Univ Montpellier

Chapitres

  • 00:04Titre
  • 00:52B Cell Amplification and Differentiation
  • 03:44Plasmablastic Cell Generation
  • 04:36Early Plasma Cell Generation
  • 05:21Long-lived Plasma Cell Generation
  • 06:29Molecular Atlas of B to PC Differentiation
  • 07:33Results: Epigenetic Factor Expression in Normal Plasma Cell Differentiation
  • 09:01Conclusion

Summary

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Traduction automatique

Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation model.

Tags

In Vitro DifferentiationHuman Normal Memory B CellsLong-lived Plasma CellsPlasma Cell DifferentiationB2 Plasma Cell DifferentiationTranscriptional ChangesPhenotypeBioinformatic ToolsMemory B Cell PurificationCD40 LigandAnti-polyhistidine Monoclonal Antibody

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