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Journal
/
Immunologie et infection
/
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
Journal JoVE
Immunologie et infection
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Journal JoVE
Immunologie et infection
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
DOI:
10.3791/58929-v
•
10:26 min
•
January 20, 2019
•
Michel Jourdan
,
Hugues de Boussac
,
Elena Viziteu
,
Alboukadel Kassambara
,
Jérôme Moreaux
2,3
1
IGH, CNRS
,
Univ Montpellier
,
2
Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology
,
CHU Montpellier
,
3
UFR de Médecine
,
Univ Montpellier
Chapitres
00:04
Titre
00:52
B Cell Amplification and Differentiation
03:44
Plasmablastic Cell Generation
04:36
Early Plasma Cell Generation
05:21
Long-lived Plasma Cell Generation
06:29
Molecular Atlas of B to PC Differentiation
07:33
Results: Epigenetic Factor Expression in Normal Plasma Cell Differentiation
09:01
Conclusion
Summary
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Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation model.
Tags
In Vitro Differentiation
Human Normal Memory B Cells
Long-lived Plasma Cells
Plasma Cell Differentiation
B2 Plasma Cell Differentiation
Transcriptional Changes
Phenotype
Bioinformatic Tools
Memory B Cell Purification
CD40 Ligand
Anti-polyhistidine Monoclonal Antibody
Article
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