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Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
DOI:

06:09 min

December 02, 2016

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Chapitres

  • 00:05Titre
  • 00:35Cell Preparation
  • 01:19Imaging
  • 02:53Image Analysis
  • 04:05Results: Behavior of Aggregation-prone Prion-like Proteins in D. discoideum during Heat Stress
  • 05:25Conclusion

Summary

Traduction automatique

The social amoebae Dictyostelium discoideum has recently been established as a system to study protein misfolding and proteostasis. Here, we describe a new imaging-based methodology to study temperature-induced protein aggregation and the cellular stress response in D. discoideum.

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