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JoVE Journal Neuroscience
Simultaneous Cryosectioning of Multiple Rodent Brains
 

Simultaneous Cryosectioning of Multiple Rodent Brains

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.

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Simultaneous Cryosectioning Rodent Brains Time-saving Method Immunohistochemical Procedures Neuroscience Studies Visualization Anatomy Local Proteins Tissue Cryosection Mount Brain Tissue Frozen Block Coronal Sections Sagittal Sections Transverse Sections Muscle Tissue Liver Tissue Transcardial Perfusion Post-fixation Paraformaldehyde Sucrose Solution Dry Ice Isopentane Glass Beaker Embedding Mold Optimal Cutting Temperature (OCT) Compound
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