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口服抗生素治疗小鼠肠道微生物群的定量聚合酶链反应分析
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
DOI:

08:33 min

November 17, 2018

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Chapitres

  • 00:04Titre
  • 00:39Administration of Antibiotics
  • 02:29Collection of Fecal Samples from Stool, Ileum Content and Ileum Wall
  • 05:16Quantification of Intestinal Microbiota by qPCR
  • 06:33Results: Quantification of Intestinal Microbiota by qPCR after Oral Antibiotic Treatment
  • 07:51Conclusion

Summary

Traduction automatique

在这里, 我们提供了详细的方案, 口服抗生素给小鼠, 收集粪便样本, dna 提取和定量的粪便细菌的 qpcr。

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