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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
Chapitres
- 00:04Titre
- 00:28Extracellular Protein Microarray Printing
- 01:55Preparation of Multivalent Bait Complexes
- 03:09Extracellular Protein Microarray Screening
- 04:36Results: Evaluation of Extracellular Protein Microarray Slide Printing and Screen of an Orphan Query Protein
- 05:11Conclusion
Here, we present a protocol to screen extracellular protein microarrays for identification of novel receptor-ligand interactions in high throughput. We also describe a method to enhance detection of transient protein-protein interactions by using protein-microbead complexes.
