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Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics
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Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

DOI:
10.3791/58296-v

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09:54 min

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September 10, 2018

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, ,
1Department of Chemical Engineering,University of Utah, 2Huntsman Cancer Institute,University of Utah

Chapitres

  • 00:04Titre
  • 00:45Fabrication of the MY133 Microchannel
  • 06:50Testing and Use of the MY133-V2000 Device
  • 07:57Results: Few if any Artifacts are Observed Near the Microchannel-solution Interface with or without Cells in Close Proximity to the Channel Wall
  • 08:45Conclusion

Summary

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Traduction automatique

This protocol describes the fabrication of microfluidic devices from MY133-V2000 to eliminate artifacts that often arise in microchannels due to the mismatching refractive indices between microchannel structures and an aqueous solution. This protocol uses an acrylic holder to compress the encapsulated device, improving adhesion both chemically and mechanically.

Tags

Refractive Index MatchingMicrofluidicsMicroscopyPDMSMY-133-V-2000UV CuringAcrylicGlass SubstratePDMS Spin CoatingBiomedical Devices

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