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开放补丁夹具移液器测量未搅拌边界层中的离子浓度: 流体流动对离子通道控制的启示
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Biochimie
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Journal JoVE Biochimie
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
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开放补丁夹具移液器测量未搅拌边界层中的离子浓度: 流体流动对离子通道控制的启示

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05:42 min

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January 07, 2019

DOI:

10.3791/58228-v

DOI:
10.3791/58228-v

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05:42 min
January 07, 2019

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2 Views
, , , , ,
1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science,Konkuk University School of Medicine, 2Department of Emergency Medical Services,Eulji University, 3Department of Mechanical Engineering,Sungkyunkwan University

Summary

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利用膜片钳记录, 从流体流动剪切力敏感性的角度研究了机械敏感离子通道。然而, 根据实验方案, 离子通道流体流动规律的结果可能是错误的。在这里, 我们提供了防止和纠正此类错误的方法, 并提供了理论依据。

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Kim, J. G., Park, S. W., Shin, K. C., Kim, B., Byun, D., Bae, Y. M. Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow. J. Vis. Exp. (143), e58228, doi:10.3791/58228 (2019).

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