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Erkennung von niedrigen Kopie Nummer integrierte virale DNA gebildet durch In-vitro- Hepatitis B-Infektion
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
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Erkennung von niedrigen Kopie Nummer integrierte virale DNA gebildet durch In-vitro- Hepatitis B-Infektion

17,894 Views

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11:14 min

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November 07, 2018

DOI:

10.3791/58202-v

DOI:
10.3791/58202-v

•

11:14 min
November 07, 2018

•

3 Views
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1Department of Infectious Diseases, Molecular Virology,Heidelberg University Hospital, 2Gastroenterology and Liver Research Laboratory,Ingham Institute, 3German Center for Infection Research (DZIF)

Summary

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Wir beschreiben hier die in-vitro- Erzeugung von HBV-DNA über eine Hepatitis B-Virus-Infektion-System und die hochempfindliche Erkennung seiner (1 – 2 Kopien)-Integration mit Inverse nested PCR.

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Cite this Article

Tu, T., Urban, S. Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection. J. Vis. Exp. (141), e58202, doi:10.3791/58202 (2018).

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