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Immunologie et infection

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Generera rekombinant aviär Herpesvirus vektorer med CRISPR/Cas9 gen redigering

Journal JoVE
Immunologie et infection

This content is Free Access.

Journal JoVE Immunologie et infection

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

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Generera rekombinant aviär Herpesvirus vektorer med CRISPR/Cas9 gen redigering

12,364 Views

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12:21 min

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January 07, 2019

DOI:

10.3791/58193-v

DOI:

10.3791/58193-v

•

12:21 min

January 07, 2019

•

4 Views

Na Tang², Yaoyao Zhang, Miriam Pedrera, Pengxiang Chang, Susan Baigent, Katy Moffat, Zhiqiang Shen, Venugopal Nair, Yongxiu Yao

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Tags

CRISPR/Cas9 Recombinant Avian Herpesvirus Viral Antigens HVT Genome Recombinant Vaccines GFP Expression Cassette Cre-LoxP System Cell Sorting Chick Embryo Fibroblasts Transfection HVT Virus Stock Plaque Forming Units Trypsin-EDTA Cell Suspension Cell Density Cell Sorter GFP-expressing Cells

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