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In Vitro Differentiering af mus granulocyt-makrofag-koloni-stimulerende faktor (GM-CSF)-producerer T hjælper (THGM) celler
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Journal JoVE Immunologie et infection
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

In Vitro Differentiering af mus granulocyt-makrofag-koloni-stimulerende faktor (GM-CSF)-producerer T hjælper (THGM) celler

DOI:
10.3791/58087-v

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10:27 min

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September 10, 2018

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, ,
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine,National University of Singapore

Chapitres

  • 00:04Titre
  • 01:01Murine Splenocyte Preparation
  • 02:10CD4+ T Cell Magnetic Bead Separation
  • 03:48Naïve (CD25-CD44loCD62Lhi) CD4+ T Cell Fluorescence-activated Cell Sorting (FACS) and Granulocyte Macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cell Differentiation
  • 05:48Mouse THGM Cell Analysis
  • 07:35Results: THGM Cytokine Profile Analysis
  • 09:30Conclusion

Summary

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Traduction automatique

Vi præsenterer her, en protokol for at differentiere murine granulocyte-macrophage-colony-stimulating-factor-producing T hjælper (THGM) celler fra naiv CD4 + T celler, herunder isolering af naive CD4 + T celler, differentiering af THGM, og analyse af differentierede THGM celler. Denne metode kan anvendes til undersøgelser af forordningen og funktion af THGM celler.

Tags

CD4 T-cellsGM-CSFT Helper CellsTHGM CellsIn Vitro DifferentiationInflammatory DiseasesMultiple SclerosisCell IsolationCell CultureMagnetic Separation

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