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复制集方法: 一种高通量方法定量测量秀丽线虫寿命

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Génétique

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Journal JoVE Génétique

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

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复制集方法: 一种高通量方法定量测量秀丽线虫寿命

DOI:

10.3791/57819-v

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11:58 min

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June 29, 2018

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Adam B. Cornwell, Jesse R. Llop, Peter Salzman³, Juilee Thakar, Andrew V. Samuelson

¹Department of Biomedical Genetics,University of Rochester Medical Center, ²Department of Biostatistics and Computational Biology,University of Rochester Medical Center, ³Non-Clinical Statistics,Bristol-Myers Squibb, ⁴Department of Microbiology and Immunology,University of Rochester Medical Center

Chapitres

00:04Titre
00:51Replica Set Method for Scoring C. elegans Longevity: Preparing the Plates
03:33Replica Set Method for Scoring C. elegans Longevity
06:33Traditional Method for Scoring C. elegans Longevity
09:48Results: Sensitivity and Application of Replica Set Lifespan Analysis
11:13Conclusion

Summary

Traduction automatique

在这里, 我们描述了复制集方法, 一种定量测量C. 线虫寿命/生存和 healthspan 的方法, 以高通量和健壮的方式, 从而允许筛选许多条件而不牺牲数据质量。该协议详细介绍了策略, 并提供了用于分析副本集数据的软件工具。

Tags

Caenorhabditis Elegans Lifespan Aging Genetic Relationships High-throughput Replica Set Method RNAi Sub-library 96-well Plate Bacteria Culture LB Medium Ampicillin Tetracycline Agar Plate Deep-welled Plate Shaking Incubation Control Wells

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