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The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
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JoVE Journal Genetik
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

8,299 Views

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11:58 min

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June 29, 2018

DOI:

10.3791/57819-v

DOI:
10.3791/57819-v

•

11:58 min
June 29, 2018

•

2 Views
, , , ,
1Department of Biomedical Genetics,University of Rochester Medical Center, 2Department of Biostatistics and Computational Biology,University of Rochester Medical Center, 3Non-Clinical Statistics,Bristol-Myers Squibb, 4Department of Microbiology and Immunology,University of Rochester Medical Center

Summary

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Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.

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Cornwell, A. B., Llop, J. R., Salzman, P., Thakar, J., Samuelson, A. V. The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan. J. Vis. Exp. (136), e57819, doi:10.3791/57819 (2018).

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