Isolation of Mandibular Gland Reservoir Contents from Bornean ‘Exploding Ants’ (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector
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August 26, 2018
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Minor workers of the ant species Colobopsis explodens are dissected to isolate the wax-like content stored in their hypertrophied mandibular gland reservoirs for subsequent solvent-extraction and analysis by gas chromatography-mass spectrometry. The annotation and identification of volatile constituents using the open-source software MetaboliteDetector is also described.
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Hoenigsberger, M., Kopchinskiy, A. G., Parich, A., Hiller, K., Laciny, A., Zettel, H., Lim, L. B., Salim, K. A., Druzhinina, I. S., Schuhmacher, R. Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector. J. Vis. Exp. (138), e57652, doi:10.3791/57652 (2018).
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