Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (<em>Formicidae</em>) for Volatilome Analysis by GC-MS and MetaboliteDetector

Michaela Hoenigsberger; Alexey G. Kopchinskiy; Alexandra Parich; Karsten Hiller; Alice Laciny; Herbert Zettel; Linda B.L. Lim; Kamariah A. Salim; Irina S. Druzhinina; Rainer Schuhmacher

doi:10.3791/57652

/

Isolation of Mandibular Gland Reservoir Contents from Bornean ‘Exploding Ants’ (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector

JoVE Journal
環境学

This content is Free Access.

JoVE Journal 環境学

Isolation of Mandibular Gland Reservoir Contents from Bornean ‘Exploding Ants’ (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector

Isolation of Mandibular Gland Reservoir Contents from Bornean ‘Exploding Ants’ (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector

8,544 Views

•

11:07 min

•

August 26, 2018

DOI:

10.3791/57652-v

DOI:

10.3791/57652-v

•

11:07 min

August 26, 2018

•

2 Views

Michaela Hoenigsberger¹, Alexey G. Kopchinskiy², Alexandra Parich¹, Karsten Hiller^3,4, Alice Laciny⁵, Herbert Zettel⁵, Linda B.L. Lim⁶, Kamariah A. Salim⁷, Irina S. Druzhinina², Rainer Schuhmacher¹

¹Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln),University of Natural Resources and Life Sciences Vienna (BOKU), Austria, ²Institute of Chemical, Environmental and Biological Engineering,TU Vienna, Austria, ³Department of Computational Biology of Infection Research,Helmholtz Centre for Infection Research, Germany, ⁴Institute for Biochemistry, Biotechnology and Bioinformatics,University of Technology (TU) Braunschweig, Germany, ⁵2nd Zoological Department,Natural History Museum Vienna, Austria, ⁶Chemical Sciences,Universiti Brunei Darussalam, Brunei, ⁷Environmental and Life Sciences,Universiti Brunei Darussalam, Brunei

概要

Minor workers of the ant species Colobopsis explodens are dissected to isolate the wax-like content stored in their hypertrophied mandibular gland reservoirs for subsequent solvent-extraction and analysis by gas chromatography-mass spectrometry. The annotation and identification of volatile constituents using the open-source software MetaboliteDetector is also described.

Explore More Videos

Mandibular Gland Reservoir Volatilome Analysis GC-MS Metabolite Profiling Ant Chemical Ecology Insect Metabolomics Dissection Isolation Volatile Organic Compounds

Read Article

Isolation of Mandibular Gland Reservoir Contents from Bornean ‘Exploding Ants’ (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector

概要

Automatically generated

Explore More Videos