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Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain
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Neurosciences
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Journal JoVE Neurosciences
Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

9,184 Views

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08:23 min

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May 12, 2018

DOI:

10.3791/57391-v

DOI:
10.3791/57391-v

•

08:23 min
May 12, 2018

•

2 Views
, , , , , , , , , ,
1Department of Anesthesiology,Ohio State University College of Medicine, 2Medical Student Research Program,Ohio State University College of Medicine, 3Department of Anesthesiology and Pain Medicine,Nationwide Children’s Hospital, 4Department of Neuroscience,University of Kentucky Medical Center

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This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.

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Geyer, E. D., Shetty, P. A., Suozzi, C. J., Allen, D. Z., Benavidez, P. P., Liu, J., Hollis, C. N., Gerhardt, G. A., Quintero, J. E., Burmeister, J. J., Whitaker, E. E. Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain. J. Vis. Exp. (135), e57391, doi:10.3791/57391 (2018).

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