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Immunologie et infection

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שכפול של הוזמן, ספריית Nonredundant של Pseudomonas aeruginosa זן PA14 Transposon ההכנסה מוטציות

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Immunologie et infection

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Journal JoVE Immunologie et infection

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

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שכפול של הוזמן, ספריית Nonredundant של Pseudomonas aeruginosa זן PA14 Transposon ההכנסה מוטציות

7,905 Views

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11:35 min

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May 04, 2018

DOI:

10.3791/57298-v

DOI:

10.3791/57298-v

•

11:35 min

May 04, 2018

•

2 Views

Eliana Drenkard*¹, Rhianna M. Hibbler*¹, D. Alina Gutu, Alexander D. Eaton, Amy L. Silverio, Frederick M. Ausubel³, Bryan P. Hurley⁴, Lael M. Yonker⁴

¹Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, ²Department of Molecular Biology,Massachusetts General Hospital, ³Department of Genetics,Harvard Medical School, ⁴Department of Pediatrics,Harvard Medical School

Tags

Pseudomonas Aeruginosa PA14NR Set Transposon Mutant Library Replication Quality Control Contamination Prevention Sterile Techniques Replicator Pin Sterilization LB Agar Plates Freezer Storage

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