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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Chimie
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Journal JoVE Chimie
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

DOI:
10.3791/57297-v

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07:30 min

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March 07, 2018

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, , , ,
1Division of Proteomics and Nanobiotechnology, Science for Life Laboratory,KTH Royal Institute of Technology, 2Global Research and Development,Thermo Fisher Scientific IDD

Chapitres

  • 00:04Titre
  • 00:33Functionalization of Gold Nanoparticles (AuNPs)
  • 03:32Enhancement of Vertical Flow Paper-based Assays
  • 05:50Results: Signal Enhancement of IgG-modified AuNPs, AgNPs, SiNPs, and IONPs
  • 07:13Conclusion

Summary

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In this work, a protocol for signal enhancement of nanoprobe-based biosensing is presented. The protocol is based on the reduction of chloroauric acid onto the surface of existing nanoprobes that consist of gold, silver, silica or iron-oxide nanoparticles.

Tags

NanoparticleGold NanoparticleSignal EnhancementColorimetricPaperGlassMES BufferPEGEDCSulfo-NHSIgG AntibodySodium PhosphateSodium ChlorideBSA

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