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A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
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Chapitres
- 00:05Titre
- 00:37Transfection of HEK293 Cells with NR1-GFP Expression Plasmid
- 02:38Cell-based Immunofluorescence Assay
- 04:41Results: Anti-NMDA Receptor Autoantibodies Detected in the Blood of Patients with Autoimmune Encephalitis
- 06:12Conclusion
We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.
