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Biologie du développement
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Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
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Biologie du développement
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Journal JoVE Biologie du développement
Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

DOI:
10.3791/56670-v

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12:06 min

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November 18, 2017

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,
1School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine,Washington State University

Chapitres

  • 00:00Titre
  • 02:02Mice, Mating, and Assessing Copulatory Plug
  • 03:25Preparation Before Tissue Collection
  • 04:40Tissue Collection
  • 07:11Measurement of Semen Liquefaction from the Uterine Content Collection
  • 09:25Résultats
  • 10:02Example of Downstream Application: Video Imaging of Sperm Motility
  • 11:13Conclusion

Summary

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Semen liquefaction is required for sperm to be liberated from the seminal gel. This study provides the procedures for collecting semen from the female reproductive tract post-coitus, and measuring semen liquefaction time.

Tags

Post-mating SemenSemen LiquefactionMiceFemale Reproductive TractSemen CollectionSperm MotilityProstate Specific Antigen (PSA)InfertilityVaginal PlugLeibovitz’s L-15 MediaFetal Bovine Serum

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