Semen liquefaction is required for sperm to be liberated from the seminal gel. This study provides the procedures for collecting semen from the female reproductive tract post-coitus, and measuring semen liquefaction time.
Li, S., Winuthayanon, W. Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice. J. Vis. Exp. (129), e56670, doi:10.3791/56670 (2017).