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激光微辐照在哺乳动物细胞单、双链断裂修复中的应用
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September 05, 2017
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共焦荧光显微镜和激光微辐照提供的工具, 诱导 dna 损伤和监测的反应, dna 修复蛋白在选定的 sub-nuclear 地区。这项技术大大提高了我们对损伤检测、信号和招聘的认识。这份手稿演示了这些技术, 检查单和双链断裂修复。
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Holton, N. W., Andrews, J. F., Gassman, N. R. Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells. J. Vis. Exp. (127), e56265, doi:10.3791/56265 (2017).
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