Journal
/
Immunologie et infection
/
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

7,233 Views

•

08:43 min

•

June 24, 2017

DOI:

10.3791/55997-v

DOI:
10.3791/55997-v

•

08:43 min
June 24, 2017

•

4 Views
, , , , ,
1Laboratory of Cellular & Molecular Biology, National Cancer Institute,National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute,National Institutes of Health

Summary

Automatically generated

We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.

Explore More Videos

T CellsMadSTORMSuper-resolution ImagingMultiplexed ImagingSingle Molecule Localization MicroscopyAlexa 647Poly-L-lysineFluorescent NanodiamondsAnti-CD3 AntibodyJurkat T CellsHBS Solution

Read Article

Cite this Article

Yi, J., Manna, A., Barr, V. A., Hong, J., Neuman, K. C., Samelson, L. E. Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM. J. Vis. Exp. (124), e55997, doi:10.3791/55997 (2017).

Download .ris file

Copy

Share Video

.

Copy