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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

7,233 Views

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08:43 min

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June 24, 2017

DOI:

10.3791/55997-v

DOI:
10.3791/55997-v

•

08:43 min
June 24, 2017

•

4 Views
, , , , ,
1Laboratory of Cellular & Molecular Biology, National Cancer Institute,National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute,National Institutes of Health

Summary

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We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.

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Cite this Article

Yi, J., Manna, A., Barr, V. A., Hong, J., Neuman, K. C., Samelson, L. E. Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM. J. Vis. Exp. (124), e55997, doi:10.3791/55997 (2017).

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