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In Vitro Polymerization of F-actin on Early Endosomes
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August 28, 2017
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Early endosome functions depend on F-actin polymerization. Here, we describe a microscopy-based in vitro assay that reconstitutes the nucleation and polymerization of F-actin on early endosomal membranes in test tubes, thus rendering this complex series of reactions amenable to biochemical and genetic manipulations.
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Muriel, O., Scott, C. C., Larios, J., Mercier, V., Gruenberg, J. In Vitro Polymerization of F-actin on Early Endosomes. J. Vis. Exp. (126), e55829, doi:10.3791/55829 (2017).
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