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In Vitro Polymerization of F-actin on Early Endosomes
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JoVE Revista Bioquímica
In Vitro Polymerization of F-actin on Early Endosomes

In Vitro Polymerization of F-actin on Early Endosomes

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12:15 min

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August 28, 2017

DOI:

10.3791/55829-v

DOI:
10.3791/55829-v

•

12:15 min
August 28, 2017

•

6 Views
, , , ,
1Department of Biochemistry,University of Geneva, 2Department of Fundamental Microbiology,University of Lausanne

Summary

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Early endosome functions depend on F-actin polymerization. Here, we describe a microscopy-based in vitro assay that reconstitutes the nucleation and polymerization of F-actin on early endosomal membranes in test tubes, thus rendering this complex series of reactions amenable to biochemical and genetic manipulations.

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Muriel, O., Scott, C. C., Larios, J., Mercier, V., Gruenberg, J. In Vitro Polymerization of F-actin on Early Endosomes. J. Vis. Exp. (126), e55829, doi:10.3791/55829 (2017).

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