Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.
Wang, J., Anguera, M. C. In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells. J. Vis. Exp. (121), e55268, doi:10.3791/55268 (2017).