Journal
/
Biologie du développement
/
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

11,115 Views

•

10:39 min

•

April 22, 2017

DOI:

10.3791/55213-v

DOI:
10.3791/55213-v

•

10:39 min
April 22, 2017

•

1 Views
, , , ,
1Laboratory of Genetics,University of Wisconsin-Madison, 2Department of Biological Sciences,Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Summary

Automatically generated

An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.

Explore More Videos

In Vitro Oocyte MaturationZebrafishMaternal Gene ProductsBiologie du développementFertilizationViable EmbryosMating TankLeibovitz’s L-15 MediumDHPGentamicinTricaineOocyte DissectionLight Cycle

Read Article

Cite this Article

Welch, E. L., Eno, C. C., Nair, S., Lindeman, R. E., Pelegri, F. Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish. J. Vis. Exp. (122), e55213, doi:10.3791/55213 (2017).

Download .ris file

Copy

Share Video

.

Copy