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Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

11,115 Views

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10:39 min

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April 22, 2017

DOI:

10.3791/55213-v

DOI:
10.3791/55213-v

•

10:39 min
April 22, 2017

•

1 Views
, , , ,
1Laboratory of Genetics,University of Wisconsin-Madison, 2Department of Biological Sciences,Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Summary

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An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.

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Welch, E. L., Eno, C. C., Nair, S., Lindeman, R. E., Pelegri, F. Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish. J. Vis. Exp. (122), e55213, doi:10.3791/55213 (2017).

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