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A蛋白制备方法在蛋白质与核余子交互使用LC-MS / MS分析的高通量鉴定

Journal JoVE
Biochimie

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Journal JoVE Biochimie

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

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A蛋白制备方法在蛋白质与核余子交互使用LC-MS / MS分析的高通量鉴定

8,176 Views

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05:43 min

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January 24, 2017

DOI:

10.3791/55077-v

DOI:

10.3791/55077-v

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05:43 min

January 24, 2017

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10 Views

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto, Hidesato Ogawa, Hiroaki Taniguchi^4,5

¹Graduate School of Frontier Biosciences,Osaka University, ²Department of Biotechnology and Genetic Engineering,Jahangirnagar University, ³Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences,Doshisha University

Tags

Protein Preparation High-throughput Protein Interactions Nuclear Cofactor LC-MS/MS Analysis Transcriptional Mechanisms Gene Regulation Flag-tagged ARIP4 HEK 293 Cells Cell Harvesting Cell Lysis Immunoprecipitation Protein Elution Mass Spectrometry

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