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بروتين طريقة التحضير لتحديد إنتاجية عالية من البروتينات التفاعل مع العامل المساعد النووية عن طريق LC-MS / MS تحليل

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Biochimie

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Journal JoVE Biochimie

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Please note that all translations are automatically generated. Click here for the English version.

بروتين طريقة التحضير لتحديد إنتاجية عالية من البروتينات التفاعل مع العامل المساعد النووية عن طريق LC-MS / MS تحليل

8,176 Views

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05:43 min

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January 24, 2017

DOI:

10.3791/55077-v

DOI:

10.3791/55077-v

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05:43 min

January 24, 2017

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10 Views

Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto, Hidesato Ogawa, Hiroaki Taniguchi^4,5

¹Graduate School of Frontier Biosciences,Osaka University, ²Department of Biotechnology and Genetic Engineering,Jahangirnagar University, ³Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences,Doshisha University

Tags

Protein Preparation High-throughput Protein Interactions Nuclear Cofactor LC-MS/MS Analysis Transcriptional Mechanisms Gene Regulation Flag-tagged ARIP4 HEK 293 Cells Cell Harvesting Cell Lysis Immunoprecipitation Protein Elution Mass Spectrometry

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