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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
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February 03, 2018
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1Centre d’Etudes Nucléaires Bordeaux Gradignan (CENBG),Université de Bordeaux, 2Centre d’Etudes Nucléaires Bordeaux Gradignan (CENBG),CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB),CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB),Université de Bordeaux
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We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.
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Muggiolu, G., Simon, M., Lampe, N., Devès, G., Barberet, P., Michelet, C., Delville, M., Seznec, H. In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis. J. Vis. Exp. (132), e55041, doi:10.3791/55041 (2018).
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