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Innsamling av serum-og mater-fri Mouse Embryonic Stem Cell kondisjonerte Medium for en Cell-fri tilnærming
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1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine,Yeungnam University, 2Physiology and Experimental Medicine Program,The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology,University of Toronto
Chapitres
- 00:05Titre
- 00:49Mouse Embryonic Stem Cell (mESC) Culture
- 02:06Serum- and Feeder-free Conditioned Medium Collection
- 03:17Senescence-associated (SA) Beta Galactosidase Assay
- 04:46Cell Cycle and qRT-PCR Analyses
- 07:19Results: Characterization of the Effects of mESC-conditioned Medium on Cell Growth, Cycle Stage, and Function
- 08:29Conclusion
Denne protokollen gir en fremgangsmåte for innsamling av muse-embryonale stamceller (Mesc) -conditioned medium (Mesc-CM) avledet fra serum (føtalt bovint serum, FBS) - og mater (mus embryonale fibroblaster, MEFs) -fri forhold for en celle -gratis tilnærming. Det kan være aktuelt for behandling av aldring og aldring-assosierte sykdommer.
