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Developmental Biology

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JoVE Journal Developmental Biology
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
 

Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach

Article DOI: 10.3791/55035-v • 09:32 min • January 8th, 2017
January 8th, 2017 •
1, 2,3, 1
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, 2Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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résumé
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This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.

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Serum-free Feeder-free Mouse Embryonic Stem Cells Conditioned Medium Biological Functions Anti-senescence Activity Soluble Factors Therapeutic Approach Age-related Diseases Research Professor Mouse Embryonic Fibroblast MEF Medium Mitomycin C Cell Culture Dish Trypsin EDTA Centrifugation Pellet Inactivated MEFs Culture Dish
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