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Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
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09:32 min
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January 08, 2017
DOI:
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine,Yeungnam University, 2Physiology and Experimental Medicine Program,The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology,University of Toronto
Summary
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This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.
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Cite this Article
Bae, Y., Sung, H., Kim, J. Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach. J. Vis. Exp. (119), e55035, doi:10.3791/55035 (2017).
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