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Optimization of a Quantitative Micro-neutralization Assay
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Optimization of a Quantitative Micro-neutralization Assay

Optimization of a Quantitative Micro-neutralization Assay

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10:09 min

December 14, 2016

DOI:

10:09 min
December 14, 2016

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Summary

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This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.

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