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Optimization of a Quantitative Micro-neutralization Assay
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December 14, 2016
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This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.
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Lin, Y., Gu, Y., McCauley, J. W. Optimization of a Quantitative Micro-neutralization Assay. J. Vis. Exp. (118), e54897, doi:10.3791/54897 (2016).
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