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06:28 min
September 25, 2016
DOI:
10.3791/54308-v
常规的方法以引发人多能的悬浮聚合基于心脏分化干细胞(hPSCs)的困扰与培养异质相对于聚集体的尺寸和形状。在这里,我们描述了采用微孔产生大小控制HPSC心脏推进的条件下培养聚集心脏分化一个可靠的方法。
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Bauwens, C. L., Toms, D., Ungrin, M. Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells. J. Vis. Exp. (115), e54308, doi:10.3791/54308 (2016).
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