Rearing the Fruit Fly <em>Drosophila melanogaster</em> Under Axenic and Gnotobiotic Conditions

Melinda L. Koyle; Madeline Veloz; Alec M. Judd; Adam C.-N. Wong; Peter D. Newell; Angela E. Douglas; John M. Chaston

doi:10.3791/54219

Journal

/

Biologie du développement

/

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

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Biologie du développement

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Journal JoVE Biologie du développement

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

DOI:

10.3791/54219-v

•

09:34 min

•

July 30, 2016

•

Melinda L. Koyle, Madeline Veloz, Alec M. Judd, Adam C.-N. Wong⁴, Peter D. Newell⁵, Angela E. Douglas³, John M. Chaston²

¹Department of Plant and Wildlife Sciences,Brigham Young University, ²Department of Entomology,Cornell University, ³Department of Molecular Biology and Genetics,Cornell University, ⁴Division of Infectious Diseases, Boston Children’s Hospital,Harvard Medical School, ⁵Biological Sciences,SUNY Oswego

Chapitres

00:05Titre
00:43Bacterial Culture and Diet Preparation
02:16Egg-laying Cages
03:13Egg Collection
03:55Egg Dechorionation and Sterile Diet Transfer
05:33Gnotobiotic Fly Generation
06:40Measuring CFU Load and Testing for Sterility
08:05Results: Successful Axenic Fly Rearing Confirmed with CFUs
08:49Conclusion

Summary

Traduction automatique

A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

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