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1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center,Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine,Yale University School of Medicine, 3Department of Molecular and Cellular Biology,Baylor College of Medicine
Chapitres
- 00:05Titre
- 01:12Mouse Embryo Yolk Sac (YS) or Aorta-gonad-mesonephros (AGM) Dissection
- 03:29Primary YS/AGM Tissue Digestion
- 04:37Nucleic Acid Dye- and Fluorescently Conjugated Antibody-labeling
- 06:19Results: Representative Flow Cytometric Identification of Hemogenic Endothelial Cells (EC), Hematopoetic Stem and Progenitor Cells (HSPC), and Non-hemogenic EC
- 07:43Conclusion
Hematopoietiska stam- och progenitorceller (HSPC) härrör från specialiserade (hemogenic) endotelceller under utveckling, men lite är känt om den process genom vilken en del endotelceller anger att bli blodbildande. Vi visar en flödescytometri baserad metod tillåter samtidig isolering av hemogenic endotelceller och HSPC från murina embryonala vävnader.
