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ワンステップの負のクロマトグラフィー精製<em>ヘリコバクター・ピロリ</emで過剰発現>好中球活性化タンパク質<em>エシェリヒア・コリ</em>バッチモードでの
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Immunologie et infection
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Journal JoVE Immunologie et infection
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
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ワンステップの負のクロマトグラフィー精製<em>ヘリコバクター・ピロリ</emで過剰発現>好中球活性化タンパク質<em>エシェリヒア・コリ</em>バッチモードでの

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10:44 min

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June 18, 2016

DOI:

10.3791/54043-v

DOI:
10.3791/54043-v

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10:44 min
June 18, 2016

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1Institute of Molecular and Cellular Biology,National Tsing Hua University, 2Department of Life Science,National Tsing Hua University

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Helicobacter PyloriNeutrophil-activating ProteinHP-NAPEscherichia ColiNegative Chromatographic PurificationBatch ModeDEAE Ion Exchange ResinProtein PurificationAggregation PreventionBradford Assay

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Kuo, T., Hong, Z., Tsai, C., Yang, Y., Fu, H. One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode. J. Vis. Exp. (112), e54043, doi:10.3791/54043 (2016).

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