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A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
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Biologie du développement
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Journal JoVE Biologie du développement
A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System

A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System

DOI:
10.3791/53955-v

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08:09 min

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May 13, 2016

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, , , ,
1Department of Cell Biology and Anatomy,School of Medicine, University of South Carolina, 2Instrumentation Resource Facility,School of Medicine, University of South Carolina, 3Biomedical Engineering Program,College of Engineering and Computing, University of South Carolina

Chapitres

  • 00:05Titre
  • 01:00Obtaining Embryos for Surgery
  • 02:42OFT Banding
  • 04:47Confirming Hemodynamic Changes
  • 06:40Results: 2D Ultrasound Data
  • 07:13Conclusion

Summary

Traduction automatique

Traduction automatique

For the first time we present here a reproducible banding procedure to alter hemodynamics in the developing heart ex ovo. This is achieved by partially constricting the outflow tract (OFT).

Tags

Ex Ovo BandingIntracardiac HemodynamicsEmbryonic Chicken SystemCardiac HemodynamicsCardiac ValvesCardiovascular DevelopmentGenetic PathwaysTyrode’s BufferOFT BandingNylon Surgical SutureEmbryo Development

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