Journal
/
发育生物学
/
A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System

A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System

DOI:
10.3791/53955-v

•

08:09 min

•

May 13, 2016

•

, , , ,
1Department of Cell Biology and Anatomy,School of Medicine, University of South Carolina, 2Instrumentation Resource Facility,School of Medicine, University of South Carolina, 3Biomedical Engineering Program,College of Engineering and Computing, University of South Carolina

Chapters

  • 00:05Title
  • 01:00Obtaining Embryos for Surgery
  • 02:42OFT Banding
  • 04:47Confirming Hemodynamic Changes
  • 06:40Results: 2D Ultrasound Data
  • 07:13Conclusion

Summary

自动翻译

自动翻译

For the first time we present here a reproducible banding procedure to alter hemodynamics in the developing heart ex ovo. This is achieved by partially constricting the outflow tract (OFT).

Tags

Ex Ovo BandingIntracardiac HemodynamicsEmbryonic Chicken SystemCardiac HemodynamicsCardiac ValvesCardiovascular DevelopmentGenetic PathwaysTyrode’s BufferOFT BandingNylon Surgical SutureEmbryo Development

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Technique to Target Microinjection to the Developing Xenopus Kidney

Gene Transfer into the Chicken Auditory Organ by In Ovo Micro-electroporation

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

In Ovo Electroporation in the Chicken Auditory Brainstem

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues

Read Article