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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
 

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Article DOI: 10.3791/53836-v • 13:24 min • April 11th, 2016
April 11th, 2016 •
1, 1, 1, 1, 1
1Asuragen, Inc.

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An integrated system for targeted next-generation sequencing of oncology specimens is described. This cross-platform system is optimized for low-quality and low-quantity tumor biopsies, accommodates low DNA inputs, includes well-characterized multi-variant controls, and features a novel variant caller that is informed by quantitative pre-analytical quality control measures.

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Keywords: Targeted Next-generation Sequencing Low-quality Tumor Biopsies Low-quantity Tumor Biopsies DNA Variant Analysis Quantification Qualification Real-time PCR DNA Template Copies Library Quantification Sample Pooling Wet And Dry Bench Processes Bioinformatics
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