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Transcriptoma Profiling de<em> In-Vivo</em> embriões produzidos pré-implantação dos bovinos por meio de duas cores Plataforma Microarray
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
DOI:

09:04 min

January 30, 2017

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Chapitres

  • 00:05Titre
  • 01:05Isolation of Total RNA
  • 03:29RNA Amplification and Labeling
  • 05:05Hybridization and Analysis
  • 07:17Results: Two-color Microarray Analysis of Day 7 Bovine Embryos
  • 08:11Conclusion

Summary

Traduction automatique

Microarray technology allows quantitative measurement and gene expression profiling of transcripts on a genome-wide basis. Therefore, this protocol provides an optimized technical procedure in a two-color custom made bovine array using Day 7 bovine embryos to demonstrate the feasibility of using low amount of total RNA.

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