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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔
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Biologie du développement
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Journal JoVE Biologie du développement
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
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鸡小脑切片的体外培养和空间上有针对性的颗粒细胞前体电穿孔

9,134 Views

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10:02 min

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December 14, 2015

DOI:

10.3791/53421-v

DOI:
10.3791/53421-v

•

10:02 min
December 14, 2015

•

3 Views
, ,
1MRC Centre of Developmental Neurobiology,King’s College London, 2School of Biological and Chemical Sciences,Queen Mary, University of London

Summary

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小脑外部颗粒层是最大的过境扩增在显影脑的部位。在这里，我们提出了一个协议，以目标基因改造这个层扩散采用体外电击和小脑切片文化从胚胎第14天鸡胚的高峰。

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Cite this Article

Hanzel, M., Wingate, R. J., Butts, T. Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors. J. Vis. Exp. (106), e53421, doi:10.3791/53421 (2015).

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