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成像免疫突触动力学内皮平面细胞模型
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Immunologie et infection
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Journal JoVE Immunologie et infection
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
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成像免疫突触动力学内皮平面细胞模型

8,789 Views

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09:25 min

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December 24, 2015

DOI:

10.3791/53288-v

DOI:
10.3791/53288-v

•

09:25 min
December 24, 2015

•

4 Views
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1Department of Medicine,Harvard Medical School – BIDMC

Summary

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Adaptive immunity is controlled by dynamic 'immunological synapses' formed between T cells and antigen presenting cells. This protocol describes methods for investigating endothelial cells both as understudied physiologic APCs and as a novel type of 'planar cellular APC model'.

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Endothelial CellsImmunological SynapseT CellsAntigen Presenting Cells (APCs)MHC/Ag-TCRF-actinAdhesion MoleculesSignaling MoleculesPlanar Cell ModelImagingAdaptive ImmunitySemi-professional APCsFluorescent Protein Reporters

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Cite this Article

Martinelli, R., Carman, C. V. An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics. J. Vis. Exp. (106), e53288, doi:10.3791/53288 (2015).

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