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Geomagnética Field (Gmf) and Plant Evolution: investigação dos efeitos dos Gmf Reversão sobre<em> Arabidopsis thaliana</em> Desenvolvimento e Expressão Gênica
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Geomagnética Field (Gmf) and Plant Evolution: investigação dos efeitos dos Gmf Reversão sobre<em> Arabidopsis thaliana</em> Desenvolvimento e Expressão Gênica
Chapitres
- 00:05Titre
- 01:14Setting of the Triaxial Coils
- 02:01Preparation of Plant Materials and Conditions for Plant Growth
- 05:10Quantitative Real Time-PCR (qPCR) Analysis of Arabidopsis Gene Expression
- 08:05Results: Effects of Geomagnetic Field Reversal on Arabidopsis Morphology, Gene Expression, and Antioxidant-related Gene Expression
- 09:23Conclusion
This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.
