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Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

DOI:
10.3791/53286-v

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11:04 min

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November 30, 2015

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, , , ,
1Department of Life Sciences and Systems Biology,University of Turin, 2Radcliffe Department of Medicine,University of Oxford, John Radcliffe Hospital

Kapitel

  • 00:05Titel
  • 01:14Setting of the Triaxial Coils
  • 02:01Preparation of Plant Materials and Conditions for Plant Growth
  • 05:10Quantitative Real Time-PCR (qPCR) Analysis of Arabidopsis Gene Expression
  • 08:05Results: Effects of Geomagnetic Field Reversal on Arabidopsis Morphology, Gene Expression, and Antioxidant-related Gene Expression
  • 09:23Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.

Tags

Geomagnetic Field (GMF)GMF ReversalArabidopsis ThalianaPlant DevelopmentGene ExpressionHelmholtz CoilsQPCRCRU3COTP1RRTF1FSD1CAT3TAPXAPX1RbohD

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