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Rapido<em> In Situ</em> Ibridazione utilizzando oligonucleotidi sonde su Paraformaldeide prefissa cervello di ratti con Sindrome serotoninergica
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Rapido<em> In Situ</em> Ibridazione utilizzando oligonucleotidi sonde su Paraformaldeide prefissa cervello di ratti con Sindrome serotoninergica
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September 23, 2015
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This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.
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Shokry, I. M., Callanan, J. J., Sousa, J., Tao, R. Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome. J. Vis. Exp. (103), e53165, doi:10.3791/53165 (2015).
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